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Automatic pipettors are extremely useful tools in laboratories that enable precise and repeatable dosing of various substances. In this article you will learn everything you need to know about automatic pipettors. We'll start with basic information about what automatic pipettors are and how they work, then move on to discuss the different types of pipettors, their applications, and practical tips for operation and maintenance.
Contents
- What are automatic pipettes?
- How do automatic pipettes work?
- Types of automatic pipettors
- Applications of automatic pipettors
- Choosing the right automatic pipettor
- How to use an automatic pipettor correctly?
- Maintenance and cleaning of automatic pipettors
- Practical tips for automatic pipettors
- Serological pipettes and Pasteur pipettes
- Summation
- FAQ
What are automatic pipettes?
Automatic pipettors are precision laboratory tools that are used to accurately dose and transfer fluids. Thanks to them, it is possible to perform precise measurements and chemical reactions, which is extremely important in many fields, such as molecular biology, analytical chemistry and medical diagnostics. Pipettors are designed to enable repeatable sample dispensing with high accuracy.
How do automatic pipettes work?
Automatic pipettors they work by sucking in and dispensing fluids. There is a piston inside the pipettor that regulates the amount of fluid taken or dispensed. To withdraw liquid, simply press the button or lever, which will draw the liquid into the pipette tip. Then, after moving the pipettor over the target location, release the button or lever, which will dispense fluid from the tip. Automatic pipettes are equipped with various functions, such as volume control, the ability to select single or multiple doses, or even programming dosing sequences.
Types of automatic pipettors
Single-channel pipettes
Single-channel pipettors are designed for dispensing single samples. They have one pipette tip and allow for precise dosing of various volumes of liquids. They are often used for work requiring high accuracy, such as preparing solutions of specific concentrations or taking samples for analysis.
Multi-channel pipettes
Multichannel pipettors have multiple pipette tips placed next to each other. This allows multiple samples to be taken or dispensed at the same time, which significantly speeds up the dosing process. Multichannel pipettors are particularly useful when multiple samples of the same volume need to be dispensed, for example for creating microplates or for multi-vessel reactions.
Electronic pipettes
They are an advanced version of automatic pipettors. They are equipped with electronic measurement and control systems, which ensures even greater precision and repeatability of dosing. Electronic pipettes often also have additional functions, such as programmable dosing sequences, memory for frequently used settings or the ability to communicate with a computer.
Applications of automatic pipettors
Laboratory pipettes are widely used in various fields where precise and repeatable dosing of liquids is crucial. Here are some examples of their applications:
Molecular biology
In molecular biology, pipetors are essential for performing various techniques such as PCR, electrophoresis and DNA sequencing. They allow for precise dosing of enzymes, DNA samples, reagents and other ingredients necessary to conduct experiments.
Analytical chemistry
In analytical chemistry, pipettors are used to prepare solutions of specific concentrations, dispense standards and samples, as well as for chemical analysis. Thanks to them, you can precisely pipette various chemical substances and perform accurate measurements.
Medical diagnostics
In medical diagnostics, pipettors are widely used to collect samples of blood, urine, saliva and other body fluids. Thanks to them, appropriate sample volumes can be precisely dosed, which is crucial in the diagnosis and monitoring of various diseases.
Laboratory work in general
Laboratory pipettes are an indispensable tool in most laboratory work. Without them, it would be difficult to perform precise measurements and chemical reactions. They are used in fields such as biochemistry, microbiology, genetics, pharmacology and many others.
Choosing the right automatic pipettor
When choosing the right pipettor, it is worth considering several factors:
Capacity
They are available in various capacity ranges, ranging from a few microliters to several milliliters. It is important to choose a pipettor with a capacity appropriate to the type of samples you will be working with.
Accuracy
Dosing accuracy is crucial, especially in laboratory work, where even small differences can make a big difference. Choose a pipettor that provides adequate dispensing accuracy for the volumes you will be using.
Repeatability
Repeatability is a feature that indicates how well an automatic pipettor copes with repeated dispensing. It is important to choose a pipettor that will provide stable and repeatable results.
Ergonomic spaces and furniture
Pipetting can be a time-consuming task, so it is worth paying attention to the ergonomic design of the pipettor. Choose a model that is comfortable to use and does not require excessive effort.
Serological pipettes used in combination with pipettors.
Serological pipettes are commonly used in laboratories to dispense and transfer various volumes of fluids. They are often used in combination with automatic pipettors, which enable precise and repeatable dosing. It is important to select serology pipettes that are compatible with the automatic pipettor you are using to ensure smooth and efficient operation.
If you are looking for high quality serological pipettesthat are compatible with automatic pipettors, we encourage you to visit www.genoplast.com. Genoplast offers various models of serological pipettes that can be used with automatic pipettors. Serological pipettes available at Genoplast are made of high-quality materials, which guarantees precise dosing and reliability in the laboratory. Before purchasing, be sure to check the compatibility of serological pipettes with the automatic pipettor you are using to ensure smooth operation and best results.
We invite you to visit www.genoplast.com and familiarize yourself with a wide range of serological pipettes that are compatible with automatic pipettors. Choose high quality and reliability to facilitate your laboratory work.
If you want to learn more about serological pipettes, please read the article Serological pipettes - everything you need to know
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How to use a laboratory pipettor correctly?
To use correctly pipetor, you need to go through a few basic steps:
- Preparing the pipettor: Before starting work, make sure the pipettor is clean and ready for use. Check that the pipette tip is clean and free from contamination. If necessary, replace the tip with a new one.
- Sample dispensing: Once the pipettor is ready, press the button or lever to aspirate the liquid. Place the pipette tip into the liquid and gently release the button or lever. The fluid will be drawn into the pipettor. Make sure the volume collected is accurate and meets protocol requirements.
- Sample Dispensing: Once you have your sample in the pipettor, move it over the target location, such as a tube, microtiter plate, or reaction vessel. Gently press the button or lever to dispense the liquid. Make sure that the liquid is discharged thoroughly and without remaining in the pipette tip.
Maintenance and cleaning of automatic pipettors
Regular maintenance and cleaning are necessary for the automatic pipettor to function properly. Here are some tips:
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- Regular cleaning: The pipette should be thoroughly cleaned after each use. Rinse the pipette tip with distilled water or a special cleaning solution, and then dry it with a paper towel or clean cloth.
- Calibration: Automatic pipettes should be calibrated regularly to ensure accuracy and repeatability. Consult your pipettor manual for instructions on how to calibrate and how often it should be performed.
- Replacing serological pipettes: Serological pipettes should be replaced regularly to avoid cross-contamination. Replace tips after each use or as recommended by the manufacturer.
Summation
Automatic pipettors are irreplaceable tools in laboratories, enabling precise and repeatable dosing of fluids. In the article, we discussed basic information about laboratory pipettors, their types, applications and practical tips for operation and maintenance. In addition, we also mentioned serological pipettes, which are available on genoplast.com. Remember that proper use of the pipettor and care for its technical condition are crucial to obtaining reliable and accurate results in laboratory work.
Also check - Guide to advanced automatic pipettes
FAQ
The frequency of pipettor calibration may vary depending on the model and manufacturer. Calibration is usually recommended every 3-12 months or as recommended by the manufacturer. Refer to your pipettor's manual to find out how often calibration should be performed.
The most common problems with pipettors are inaccurate dosing, jammed piston, contamination of pipette tips and calibration problems. If you encounter problems with your pipettor, consult the user manual or contact the manufacturer for technical assistance.
To extend the life of your pipettors, clean and maintain your pipettors regularly according to the manufacturer's recommendations. Replace pipette tips after each use, avoid mechanical damage and store pipettes in appropriate conditions. Regular calibration of pipettors also contributes to maintaining their high quality and accuracy.